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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells
doi: 10.1016/j.xpro.2020.100150
Figure Lengend Snippet: Expected Outcomes (A) Yield of a single assay. Shown are day 12 organoids distributed into 24 wells. (B) Single 24-well of the same assay. (C) High efficiency of tubule formation, shown are day 12 organoids. (D) Confocal images of immunohistological stainings on sections of day 12 organoids (derived from the MANZ-2-2 iPSC line) showing WT1 + podocytes, EPCAM + or HNF1B + tubular epithelia, LRP2 + or CUBN + proximal tubules, and CHD1 + distal tubules and connecting/collecting duct epithelia. Scale bars, 0.5 cm (B); 1 mm (C); 100 μm (D).
Article Snippet: WT1 mouse IgG1 (use 1:25).
Techniques: Derivative Assay
Journal: STAR Protocols
Article Title: Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells
doi: 10.1016/j.xpro.2020.100150
Figure Lengend Snippet:
Article Snippet: WT1 mouse IgG1 (use 1:25).
Techniques: Recombinant, Immunohistochemistry, Membrane, Gentle, Knock-Out, Plasmid Preparation, Microscopy, Staining, Sterility, Suction Filtration, Marker, Clone Assay, Mutagenesis
Journal: International Journal of Molecular Sciences
Article Title: Targeting NF-κB by the Cell-Permeable NEMO-Binding Domain Peptide Improves Albuminuria and Renal Lesions in an Experimental Model of Type 2 Diabetic Nephropathy
doi: 10.3390/ijms21124225
Figure Lengend Snippet: Cell-permeable NBD peptide decreased podocyte loss and basement membrane thickness in BTBR ob/ob mouse kidney. ( A ) Representative images of WT-1 immunohistochemistry (magnification 630×) and quantification of WT-1 + cells in glomerular cross section (gcs). Transmission electron microscopy images (magnification 9900×) and measurement of ( B ) glomerular basement membrane (GBM) and ( C ) tubular basement membrane (TBM) thickness. Data shown as scatter dot plots and mean ± SD of each group (n = 6–8 mice/group). *** p < 0.001; **** p < 0.0001 vs. diabetic vehicle control.
Article Snippet: The primary antibodies for immunodetection studies were:
Techniques: Immunohistochemistry, Transmission Assay, Electron Microscopy